C-REACTIVE PROTEIN TEST – 1 Test Kit

Description

GENERAL:

C-REACTIVE PROTEIN

C-Reactive Protein (CRP) is an acute phase protein produced primarily by the liver and increases in concentration due to injury, infection, and inflammation. Although not a specific marker for any particular condition, CRP is currently used as a general diagnostic indicator of infection and inflammation and to monitor patients' responses to treatment and postoperative recovery.
The CRP level in the blood is high in bacterial infections and low in viral infections. Therefore, C-reactive protein dosage may be a useful tool to determine the origin of an inflammatory state. Monitoring CRP levels over time can determine the effectiveness of a therapy and evaluate a patient's healing process. CRP levels in the blood reach their peak concentration between 24 and 48 hours after the onset of the first symptoms of infection/inflammation, and then begin to decline as the infection resolves/inflammation subsides. The C-Reactive Protein Test provides a semi-quantitative evaluation of blood CRP levels that aids in therapeutic diagnosis and monitoring.

PRINCIPLE OF THE TEST
The C-REACTIVE PROTEIN TEST is a rapid immunochromatographic test for semi-quantitative detection
of C-Reactive Protein in human blood samples. The test uses special gold-conjugated monoclonal antibodies integrated into the reactive strip.

MANUAL:
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TEST PROCEDURE:
1) Wash hands with soap and warm water, rinse with clean water and allow to dry. Note: The use of
warm water facilitates capillary blood collection because it induces vasodilation.
2) Place the required material as follows: open the aluminum bag, take out only the test cassette and throw it away
bag containing the desiccant. Open the plastic package containing the pipette. Open the vial by loosening the white cap
rotate it and make sure it does not fall to the ground. –fig. a
3) Gently rotate the protective cap of the sterile lancet 360° without pulling, remove the cap and
throw it away. –fig. b
4) Gently massage the finger chosen for the injection (the side of the ring finger is recommended). It is important
that the massage takes place from the palm of the hand to the fingertip, to improve blood circulation. Press it open
end of the lancet (the side where the cap is pulled out) against the fingertip -fig. C The tip of the lancet
automatically retracts after use. If the lancet does not work properly, throw it away and use the second one
supplied lancet. If the second is not necessary, it can be done without special precautions
deleted.
5) Hold the hand down and massage the finger until a large drop of blood forms. It's important to
massaging the palm to the fingertip to improve blood circulation. –fig. D
6) Take the pipette without pressing the bulb. Two sampling methods are proposed:
–fig. E1: Hold the pipette horizontally without pressing the bulb and place it in contact with the
drop of blood, which will end up in the pipette due to capillarity. Move the pipette away when the black line is
reaches. If there is not enough blood, continue to massage the finger until the black line is reached.
–fig. E2: Place the pipette on a clean, flat surface with the tip out of the shelf, then insert the
drop of blood in contact with the pipette, it will enter by capillarity. If the blood is not enough, it remains
you can massage the finger until the blood reaches the black line.
As much as possible, avoid constantly moving the pipette tip away from the finger to prevent the formation of
prevent air bubbles.
7) Place the blood collected with the pipette into the previously opened vial. Press the pipette bulb 2 or 3 times to make sure
Make sure that all the blood has ended up in the dilution solution. Place the screw cap back on the bottle and
shake well for at least 10 seconds. –fig.F
8) Unscrew the blue cap from the dropper bottle (leave the white cap firmly in place). –fig.G.
Place 3 drops in the well on the cassette (S). –fig.H
9) Wait 5 minutes and then read the results as indicated.

INTERPRETATION OF THE RESULTS
READ THE RESULT AFTER EXACTLY 5 MINUTES
DO NOT READ THE RESULT AFTER 6 MINUTES
The result must be interpreted independently of the color intensity of the bars that may appear in the display window.

• NEGATIVE, C-REACTIVE PROTEIN UNDER 10 MG/L
  A colored line only appears under the C (Control) sign, as in the figure on the right. This result
  indicates that the CRP concentration is less than 10 mg/l and indicates the absence of infection and inflammation.
• POSITIVE, C-REACTIVE PROTEIN BETWEEN 10 AND 40 MG/L
  Two colored lines appear in the display window, one at the C (Control) mark, the other at the T1 mark, as shown in the figure to the right. It is advisable to consult a doctor because CRP values ​​between 10 and 40 mg/l may be associated with a viral infection or the onset of
  of a bacterial infection. Inflammation caused by (for example) physical trauma can also lead to:
  lead to such a result.
• POSITIVE, C-REACTIVE PROTEIN BETWEEN 40 AND 80 MG/L
  Three colored lines appear in the display window, one at the C mark and two others at the T1 and T2 marks, as shown in the figure on the right. Consult a doctor as this result may be related to a viral or bacterial infection or physical trauma.
• POSITIVE, C-REACTIVE PROTEIN OVER 80 MG/L
  Four colored lines appear in the display window, one at the C mark and three others at the T1, T2 and T1 marks, as shown in the figure on the right. Consult a doctor as this result may be related to a bacterial infection or severe inflammation.

NOTE: The color intensity in the control and test line areas may differ (as shown in the images). The intensity of the bars is not relevant; only their presence must be taken into account.

INVALID RESULT:
The check mark does not appear (no lines appear or no
one or more T lines appear without the C line).
Insufficient sample volume or incorrect procedural techniques are the most likely reasons for the absence of the control line. Review the procedure and repeat the test with
a new test cassette and a new blood sample.”;}i:3;a:3:{s:5:”title”;s:3:”Q&A”;s:2:”id”;s:2:”qa ”;s:7:”content”;s:2617:”HOW DOES THE C-REACTIVE PROTEIN TEST WORK?

The test detects the presence of CRP in human whole blood samples using specific antibodies and colloidal gold particles in the test strip.

WHEN CAN THE TEST BE USED?

The C-REACTIVE PROTEIN TEST can be used in case of symptoms of infection or inflammation, such as fever, headache or weakness, or to monitor the healing process after operations and minor injuries. The test can also be used to monitor the effectiveness of therapeutic treatment and can be performed at any time of the day.

COULD THE RESULT BE WRONG?
The result is correct as long as the instructions are carefully followed and the test is stored under the conditions stated in the “Precautions” section. However, the result may not be correct if: the device comes into contact with other liquids before use, if the amount of blood and/or diluent is not sufficient, if the number of drops placed in the test well is incorrect or if the reading times are not strict are followed. With the supplied plastic pipette, the user can be sure that he has collected the correct amount of blood.

HOW SHOULD I INTERPRETE THE TEST IF THE COLOR AND INTENSITY OF THE TEST AND CONTROL BANDS ARE DIFFERENT?

The color and intensity of the lines are not important for the interpretation of the result. Please refer to the “Interpretation of the Results” section and the images to correctly evaluate the result.

IS THE RESULT RELIABLE IF IT IS READ AFTER 6 MINUTES?

No. The test should be read 5 minutes after the end of the procedure and no later than 6 minutes after this time. Results read after 6 minutes may not be correct.

WHAT SHOULD I DO IF THE RESULT IS POSITIVE?
If the result is positive and the CRP is higher than 10 mg/l, refer to the “Result interpretation” section and contact your doctor as soon as possible, as antibiotic treatment may be necessary.

WHAT SHOULD I DO IF THE RESULT IS NEGATIVE?
A negative result means that the CRP concentration is less than 10 mg/l, which means that no viral or bacterial infection is ongoing. Consult a doctor if symptoms persist.

HOW ACCURATE IS THE C-REACTIVE PROTEIN TEST?
The C-REACTIVE PROTEIN test shows high concordance with analytical reference methods, with 97% accuracy in distinguishing between normal (< 10 mg/l) and abnormal values.